Introduction to high-resolution cryo-electron microscopy.

High Resolution Imaging of Radiation Sensitive Biological Samples

Cryo-electron microscopy has seen incredible progress recently. From the first reconstruction of frozen hydrated samples to recent atomicresolution structures, structural biology is undergoing a revolution. However, until recently, the resolution of cryo-EM structures was often significantly lower than that of X-ray crystallography. We have reached a point where, for an increasing number of sam...

Mathematics for cryo - electron microscopy

Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method for biological macromolecules. Cryo-EM was selected by Nature Methods as Method of the Year 2015, large scale investments in cryo-EM facilities are being made all over the world, and the Nobel Prize in Chemistry 2017 was awarded to Jacques Dubo...

Cryo-SEM of Perpendicular Cross Freeze-Fractures Through a High-Pressure-frozen Biofilm

The cryo scanning electron microscopy (cryo-SEM) as well as high-pressure-freezing (HPF) belongs to cutting edge techniques in electron microscopy of hydrated samples [1]. However, their combination is not always easily applicable. Here, we present a way of combining high-pressure-freezing using EM PACT2 (Leica Microsystems) that fixes hydrated samples on a 1.4 mm sapphire discs and high resolu...

Cryo-EM enters a new era

Advances in detector hardware and image-processing software have led to a revolution in the use of electron cryo-microscopy to determine complex molecular structures at high resolution.

“ for developing cryo - electron microscopy for the high - resolution structure determination of biomolecules in solution ” 2017 Nobel Prize in Chemistry